Research Catalog

Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure

Title
Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure [electronic resource] / Organisation for Economic Co-operation and Development
Author
Organisation for Economic Co-operation and Development.
Publication
Paris : OECD Publishing, 2013.
Electronic Resource
Full text online available onsite at NYPL

Details

Description
10 p.
Series Statement
OECD Guidelines for the Testing of Chemicals, Section 2, 2074-5761.
Uniform Title
OECD Guidelines for the Testing of Chemicals, Section 2
Subject
ISBN
9789264203723
LCCN
10.1787/9789264203723-en
Author
Organisation for Economic Co-operation and Development.
Title
Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure [electronic resource] / Organisation for Economic Co-operation and Development
Imprint
Paris : OECD Publishing, 2013.
Series
OECD Guidelines for the Testing of Chemicals, Section 2, 2074-5761.
OECD Guidelines for the Testing of Chemicals, Section 2, 2074-5761.
Summary
This Test Guideline (TG) describes a honey bee brood acute toxicity test under laboratory conditions. The method aims at the determination of the lethal dose (72-h LD50) following single exposure of larvae to a chemical. On day 1 (D1) of the study, first instar synchronised larvae are taken from the comb of three colonies (thirty-six larvae in total per group) and individually placed into 48 well-plates where they are fed a standardized amount of artificial diet. On day 4 (D4) of the test, a single dose of the test chemical is administered to the larvae with the diet. Each group of thirty-six larvae is administered a given dose in a range of five increasing doses. Mortalities are recorded on D5, D6, and D7 of the test. The 72-hr LD50 is calculated for larvae (cumulative mortality at D7). The study report also includes a number of important elements, in particular regarding test conditions (e.g., temperature and humidity).
Connect to:
Indexed Term
Environment
Other Editions:
Essai n° 237 : Essai de toxicité larvaire chez l'abeille domestique (Apis mellifera), par exposition unique 9789264203754 fre
Other Standard Identifier
10.1787/9789264203723-en doi
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