Research Catalog

Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages

Title
Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages [electronic resource] / Organisation for Economic Co-operation and Development
Author
Organisation for Economic Co-operation and Development.
Publication
Paris : OECD Publishing, 1998.

Available Online

Full text online available onsite at NYPL

Details

Description
20 p.; 21x29.7cm.
Series Statement
OECD Guidelines for the Testing of Chemicals, Section 2, 2074-5761.
Uniform Title
OECD Guidelines for the Testing of Chemicals, Section 2
Subject
ISBN
9789264070141
LCCN
10.1787/9789264070141-en
Author
Organisation for Economic Co-operation and Development.
Title
Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages [electronic resource] / Organisation for Economic Co-operation and Development
Imprint
Paris : OECD Publishing, 1998.
Series
OECD Guidelines for the Testing of Chemicals, Section 2, 2074-5761.
OECD Guidelines for the Testing of Chemicals, Section 2, 2074-5761.
Summary
In this Short-term Toxicity Test on Fish Embryo and Sac-Fry Stages, the life stages from the newly fertilized egg to the end of the sac-fry stage are exposed. The embryo and sac-fry stages of fish are exposed to five concentrations of the test substance dissolved in water. A choice, depending on the nature of the test substance, is possible between a semi-static and a flow-through procedure. The test starts with placing at least 30 fertilised eggs divided equally between at least three replicate test chambers per concentration, and is terminated just before the yolk-sac of any larvae in any of the test chambers has been completely absorbed or before mortalities by starvation start in controls. Lethal and sub-lethal effects are assessed and compared with control values to determine the lowest observed effect concentration and hence the no observed effect concentration. Alternatively, they may be analysed using a regression model in order to estimate the concentration that would cause a given percentage effect. The study report should include: the daily counting of the offspring, the daily recording of the parent mortality, the weekly measurement of oxygen concentration, temperature, and pH values; and the determination of the concentrations of test substance. It should also include the observations of abnormal appearance, abnormal behaviour, hatching and survival...
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Indexed Term
Environment
Other Editions:
Essai n° 212: Poisson, essai de toxicité à court terme aux stades de l'embryon et de l'alevin 9789264070158 fre
Other Standard Identifier
10.1787/9789264070141-en doi
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